Table 1. Lipid composition of RAW cells subjected to various treatments.

	Polar Lipids								Apolar Lipids
Treatment of Cells:	Total Polar Lipid		Free Cholesterol		Chs		Free Chol+Chs		Cholesteryl Esters		Triacylglycerides
	24 h	48 h	24 h	48 h	24 h	48 h	24 h	48 h	24 h	48 h	24 h	48 h
Control	100±10	100±8	22±3	25±1	0	0	22±3	25±1	16±4	14±3	1.6±0.3	2.7±1.8
Ac-LDL	100±13	100±15	26±2	30±2	0	0	26±2	30±2	61±7	58±8	2.9±0.4	6.7±2.8
Chs-LDL	100±21	100±13	25±5	35±3	29±1	43±1	54±6	78±4	24±16	58±16	3.0±0.7	4.3±2.4
Chs-POPC	100±18	100±20	14±1	14±3	40±1	57±1	54±2	71±4	5±2	5±2	2.1±0.3	1.8±0.9
Native LDL	100±4		43±1		0		43±1		257±25		10.3±1.0

Polar lipids have been defined as the sum of phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, ceramides, diacylglycerides, free cholesterol and cholesteryl hemisuccinate. All values are expressed as a mol % of the total polar lipids. The apolar lipids (cholesteryl esters and triacylglycerides) are expressed as a mol % of the total polar lipid fraction. For purposes of comparison, the bottom row shows the lipid composition of Nat-LDL. Chs-LDL are Nat-LDL loaded with Chs with a Chs/LDL particle molar ratio of 1000∶1, Chs-POPC are POPC liposomes with a Chs/POPC molar ratio of 45∶55. The values in the columns entitled “Total Polar Lipids" is a reference point by definition and, therefore, always 100%. These values have only been included to show the standard deviation of the measurement since all the other values are given relative to the “Total Polar Lipid" content.