Table 2. Fatty acid composition of the cholesteryl esters extracted from RAW Cells and, for purposes of comparison, the fatty acid composition of Nat-LDL.

Fatty Acid	Nat-LDL		Ac-LDL		Chs-LDL		Extract of Nat-LDL
	24 h	48 h	24 h	48 h	24 h	48 h
14∶0	4.3±0.6	2.4±0.4	9.8±0.7	8.5±1.6	4.7±1.7	1.5±0.1	0.6±0.2
16∶0	6.3±1.8	5.0±1.7	18.2±1.3	14.2±2.2	14.1±1.7	12.9±1.0	9.1±1.2
16∶1	1.7±0.1	1.6±0.5	4.3±0.2	4.3±0.6	4.7±0.7	4.1±0.4	2.8±0.4
18∶0	2.8±1.1	1.4±0.2	9.2±0.4	7.0±0.9	3.0±1.6	1.0±0.2	0.4±0.1
18∶1	13.7±1.7	13.3±2.0	39.2±0.9	40.2±0.7	27.3±4.8	21.3±1.6	15.3±1.4
18∶2	4.5±0.3	8.7±5.9	11.1±1.3	15.8±0.7	37.8±9.2	50.6±1.1	57.1±2.5
20∶4	0.7±0.1	2.6±1.3	1.4±0.6	2.4±1.6	3.9±1.1	5.2±1.3	9.5±0.7

Quantitative lipidomic analysis of the intracellular cholesteryl esters in lipid extracts of cells incubated for 24 or 48 h with 300 µg/ml of Ac- or Chs-LDL. Only the major cholesteryl esters are represented in the table. Changes in the content of Cholesteryl oleate (18∶1) and Cholesteryl Linoleate (18∶2) are highlighted. Lipid extracts of native LDL were used as control. The cholesteryl esters distribution is shown as percentage of total cholesteryl esters. The results are the mean ± SD of three independent experiments obtained with three different LDL preparations.