Figure 2. Saponin increases the sensitivity of PMCAb for PrP^CWD detection.

CWD-positive brain homogenate (BH; 10% w/v) was diluted using serial five-fold dilutions in NBH and subjected to one round of 96-cycle PMCAb in the presence of absence of 0.045% saponin. BH, brain homogenate; MWM, molecular weight marker.