Figure 3. Fenofibrate protects against JEV infection, in vivo, in mice.
(A) Fenofibrate markedly reduced the mortality in JEV infected animals (n = 15 per group). (B) Scores depicting the reversal of behavioral deficits following fenofibrate treatment compared to JEV infection alone (n = 15 per group, *p<0.05). (C,D). Stereological analysis using immunolabeling with antibody to Iba-1 showed significant decrease in number of activated microglia following pretreatment with fenofibrate compared to JEV alone. Inset depicts the negative control for immunohistochemical localization of Iba-1, which was performed using normal rabbit IgG. (E) Pretreatment with fenofibrate decreased the JEV mediated increase in LTB4 levels in the cortex. Data is represented as (C) vehicle control, (F) fenofibrate, (J) JEV, (F+J) fenofibrate+JEV treated mouse brain cortex (n = 6 per group, *and #p<0.05). Treatment with fenofibrate alone results in decreased number of activated microglia (inset in D) and levels of LTB4 (inset in E).