Figure 4. Properties of the MDA-MB-231 cells engineered to overexpress matriptase.

(MT-SP1 A, MT-SP1 B) and the respective control clones (Empty A, Empty B). (A) Bright field images of the selected clones and parental cells. (B) Western blots performed on total cell lysates, and (C) proteins precipitated from conditioned medium, using matriptase specific antibody. (D) In vitro growth curves for the selected clones and the parental cell line. There were no significant differences between the clones (p>0.05). (E) Representative fluorescence images of MT-SP1 B and Empty A cells stained with fluorescein-labelled phalloidin (actin cytoskeleton) and DAPI (nuclei). Similar results were obtained with MT-SP1 A and Empty B clones. Scale bar 10 µm.