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. 2001 May 1;98(10):5608–5613. doi: 10.1073/pnas.091101898

Figure 5.

Figure 5

Antibody antimaspin effect on ENaC β-subunit expression in 106/E41–88 cells. RT-PCRs were performed with the amiloride-sensitive ENaC β-subunit-specific primers by using cDNA of uninduced and DMSO-induced 106A10 cells (lanes 1 and 2), uninduced and DMSO-induced LA7 cells (lanes 3 and 4), uninduced 106E41–88 rat8-transfected cells exposed to antimaspin antibody (lane 5), and uninduced 106E41–88 not exposed to the antimaspin antibody (lane 6). Lane 7, PCR-negative control. A PCR, using the same primers, was performed on the same samples after DNase treatment and before retrotranscription (lanes 8–13).