Figure 5. Enlarged areas of mature Alcian-blue stained goblet cells are detected in agr2-overexpressed embryos.

Substantially increased areas of mature Alcian blue-stained intestinal goblet cells in 42% 104-hpf agr2-overexpressed (C, n = 29) embryos compared to lacZ-overexpressed (B, n = 20) embryos and wild type (A, n = 18) embryos were observed. Comparison of the area of Alcian blue-stained goblet cells in wild type, agr2- and lacZ-overexpressed embryos is shown (D). Arrows indicate examples of Alcian-blue stained goblet cells with enlarged areas. Student's t-test was conducted and *p<0.001. Scale bars represent 100 µm.