(A) Expression level of members of the UPR pathway (hspa5, xbp1s, atf4b1, chop) was not altered in agr2 morphants when normalized to wild type or to agr2–5 mmMO1 and 5 mmMO2-coinjected 104-hpf embryos by qPCR. (B) Expression of members of the UPR pathway (hspa5, xbp1s, atf4b1, chop) was not altered in agr2-overexpressed 104-hpf embryos when normalized to wild type or to lacZ-overexpressed embryos by qPCR. (C) agr2 expression level was not significantly induced upon 3 µg/ml tunicamycin treatment by qPCR. One-way analysis of variance and Tukey's honestly significant different method (T-method) were conducted among different genes after treatment with different concentrations of tunicamycin [27]. Different letters (a, b, c) indicate a statistically significant difference (p<0.05). Average gene expression in fold changes for respective gene is indicated. Error bars indicate the standard error.