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. 2012 Apr 13;7(4):e34012. doi: 10.1371/journal.pone.0034012

Figure 4. RT-PCR analysis of HOXC8 in different tissues in various chicken breeds.

Figure 4

(A) RT-PCR analysis in skin samples from the progeny of a heterozygous Cr/cr+ chicken. (B) RT-PCR of seven tissues from Crested and wild-type Silkie chickens. Samples were collected from two individuals at 12 weeks of age. -RT indicates a reaction without reverse transcriptase. CK indicates negative control. (C) RT-PCR results for the cranial skin in adult individuals of 26 Chinese local chicken breeds. Breeds are as follows: 1. Lu Yuan; 2. Gu Shi; 3. Hu Xu; 4.Chinese Fatty Chicken; 5. Xian Ju; 6. Da Gu; 7. Silkie; 8. Dou Chicken; 9. Green Egg Chicken; 10. Wen Chang; 11. You Xi Ma; 12. Ai Jiao Huang; 13. Shou Guang; 14. An Ka; 15. Bian Chicken; 16. White Ear; 17. Kuai Da Silkie; 18. Cha Hua; 19. Yin Xing Bai; 20. Chong Ren Ma; 21. Wa Hui; 22. Jin Hu Wu; 23. Tibet Chicken; 24. Shi Qi Za; 25. Black Lang Shan; 26. Qing Yuan Ma; CK: Negative control. Numbers in red indicate chicken breeds with Crest phenotype and black numbers wild-type breeds (Table S12).