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. 2012 Apr 13;7(4):e34424. doi: 10.1371/journal.pone.0034424

Figure 1. Expression studies of full length and truncated MLN64 protein.

Figure 1

A) MLN64 is a late endosomal protein that consists of four transmembrane segments and a cytosolic START domain. B) Schematic of full length and truncated MLN64 proteins containing only the transmembrane domains (TM) or the cytosolic START domain (START). C) Expression of MLN64, TM or START (red signal) causes accumulation of free cholesterol (blue signal) in the E/L system but only MLN64 and TM colocalize in late endosomes with CD63-YFP (green signal), whereas START displays an expression pattern characteristic of the ER. D) Cells expressing MLN64 and TM, but not START, display enlarged endosomal vesicles at 24 hours post-transfection, and this phenotype becomes more pronounced after 72 hours.