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A) Western blot analysis of endogenous MLN64 from cell lysates bound to purified GST-tagged 14-3-3 isoforms indicates interactions between MLN64 and 14-3-3 γ, ε, η, ζ, τ and σ isoforms. ε* denotes a 14-3-3 mutant that cannot bind target proteins. B) Western blot analysis of purified Flag-tagged MLN64 bound to purified 14-3-3-GST isoforms indicates the strongest interactions with the 14-3-3 η and τ isoforms.
