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. 2012 Apr 13;7(4):e34735. doi: 10.1371/journal.pone.0034735

Figure 4. Humoral immune response observed in IFNAR(−/−) mice vaccinated with heterologous prime boost vaccination with pcDNA3 and rMVA expressing VP2/VP7/NS1.

Figure 4

The presence of antibodies specific of VP2, VP7, and NS1 in serum of immunized (A) and non-immunized (B) IFNAR(−/−) mice was analyzed by ELISA. Sera from mice immunized with pcDNA3-VP2,-VP7,-NS1 (day 0) and rMVA-VP2,-VP7,-NS1 (day 14) and non-immunized were collected at days 0, 14, and 28 before the challenge with BTV-4, and dilution 1∶50 was analyzed by ELISA as described in Materials and Methods. Standard deviations are shown as error bars. (C) BTV-4 neutralizing antibody detection in VP2/VP7/NS1 immunized mice by VNT. Neutralization titers at day 28 in sera of animals immunized with pcDNA3-VP2,-VP7,-NS1 (day 0) and rMVA-VP2,-VP7,-NS1 (day 14) (▪) and non-immunized (•) are shown. Means are presented (⁃) and standard deviations are shown as error bars.