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. 2012 Apr 13;7(4):e34735. doi: 10.1371/journal.pone.0034735

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Calvo-Pinilla et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sera from mice immunized with pcDNA3-VP2,-VP7,-NS1 (day 0) and rMVA-VP2,-VP7,-NS1 (day 14) (solid symbols) or pcDNA3 (day 0) and MVA (day 14) (symbols) were collected at days 0, 14, 21, and 28. The levels of cytokines were evaluated by a multiplex fluorescent bead immunoassay for quantitative detection of 5 mouse cytokines (Millipore's MILLIPLEX Mouse Cytokine kit). Samples were analyzed with a Luminex 2010 (Luminex Corporation). Means are presented (⁃).