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. 2012 Apr 13;7(4):e34735. doi: 10.1371/journal.pone.0034735

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Calvo-Pinilla et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunofluorescence microscopy using a mouse polyclonal antibody against BTV-4 in BHK-21 cells transfected with pcDNA3-VP2, pcDNA3-VP7, pcDNA3-NS1 and different combinations of these plasmids. (B) Immunofluorescence microscopy using a mouse polyclonal antibody against BTV-4, or monoclonal antibodies against VP2 and VP7 from BTV-4 in BHK-21 cells transfected with pcDNA3-VP2,-VP7,-NS1.