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. 2012 Apr 13;7(4):e34633. doi: 10.1371/journal.pone.0034633

Figure 4. Lcn2 transcription requires new protein synthesis, but C/ebpδ does not. C/EBPδ is recruited to the promoter of LCN2 upon LPS stimulation in an IRAK-1 dependent manner.

Figure 4

(A) Cyclohexamide, a protein synthesis inhibitor, blocks Lcn2 transcription upon LPS stimulation. (B) The induction of C/ebpδ does not require new protein synthesis. (B) Wild-type kidney fibroblasts were untreated (DMSO) or pretreated with cyclohexamide 5 ug/mL for 1 hour. Immediately following pretreatment, the cells were stimulated with or without LPS for 4 hours. Transcripts were quantitated using qRT-PCR and standardized using Gapdh as the internal loading control. Each experiment was performed in triplicate. *P<0.05 (C) The recruitment of C/EBPδ to the promoter of LCN2 upon LPS stimulation depends upon IRAK-1. Nuclear lysates were subject to ChIP analysis using a C/EBPδ specific antibody. The arrow points to the specific amplification signal for C/ebpδ promoter. The * sign indicates a non-specific amplification.