Fig. 4.

Distribution of the Hb expression boundary location. Three different nuclear densities were simulated: low (a), medium (b) and high (c) consisting of 26, 51, and 101 nuclei, respectively. Each nuclear density simulation was repeated 60 times, from which distributions were extracted. Low nuclear density decoding is more precise (85% of the cases produce same boundary location) than high nuclear density decoding (51.6% reproducibility), but accounts for larger errors. Medium nuclear density is intermediate with respect to both of these criteria