Table 1.

Primers and Probes for gene expression in CD133 and VEGF, VEGFR-1, -2,- 3 and genotyping in CD133

Gene	Gen Bank accession	Forward (5′-3′)	Reverse (5′-3′)	Taqman probe (5′-3′)
β-actin	NM_001101	GAGCGCGGCTACAGTT	TCCTTAATGTCACGCACGATTT	ACCACCACGGCCGAGCGG
CD133	NM_006017	CAAGGACAAGGCGTTCACAG	GTTGGGTCTCAGTCGGTCAA	TTCCGCCTCCTAGCACTGAATTGA
VEGF	NM_003376	AGTGGTCCCAGGCTGCAC	TCCATGAACTTCACCACTTCGT	ATGGCAGAAGGAGGAGGGCAGAATCA
VEGFR1	NM_002019	CGCATATGGTATCCCTCAACCT	AGTCACACCTTGCTTCGGAATG	TGGTTCTGGCACCCCTGTAACCATAA
VEGFR2	NM 002253	CCTGTGGCTCTGCGTGGA	CTGAGCCTGGGCAGATCAAG	CACTAGGCAAACCCACAGAGGCGGC
VEGFR3	NM_182925	GGACACCCTGCAAGATGTTTG	TCACGGCACTGTGGCATGA	CGCCGCCGGAGACTACGCTGG
Polymorphism RS number	Annealing temperature	Forward (5′-3′)	Reverse (5′-3′)	Digesting enzyme
rs3130	60°	AGAACTGCAATCTGCACATGA	TGATCAGCAATGAAGAACTGG	EcoR1
rs2286455	60°	ACGCCTCTTTGGTCTCCTTG	TCCATCCCAAGTCCCTTTAG	Mbo1
rs2240688	60°	TCAAGATCTCTCTCTCTCTTTTGA	GTGGAACATGGCCAATCTTT	Cvikl-1