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. 2001 May 1;98(10):5619–5624. doi: 10.1073/pnas.091502398

Figure 3.

Figure 3

Cholesterol dependence of raft association of SNARE proteins, and analysis of SNARE association with Lubrol-insoluble rafts. PC12 cells were solubilized in (A) either 1% Triton X-100 (control) or 0.5% Triton + 0.5% saponin (+ saponin); or (B) 1% Triton X-100 (Triton) or 1% Lubrol WX (Lubrol), and fractionated on a discontinuous sucrose gradient, as detailed in Materials and Methods. Equal volumes of the recovered fractions were separated by SDS/PAGE and transferred to nitrocellulose for immunoblotting analysis by using antibodies specific for syntaxin 1A, SNAP-25 and VAMP2.