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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Kidney Int. 2012 Jan 25;81(9):880–891. doi: 10.1038/ki.2011.469

Figure 5. Ectopic expression of Id1 potentiates RANTES induction in tubular epithelial cells and promotes monocyte chemotaxis.

Figure 5

(a, b) Id1 potentiates RANTES expression in response to TNF-α stimulation. HKC-8 cells transfected with either Id1 expression vector or pcDNA3 empty plasmid were treated with different doses of TNF-α for 24 h as indicated. Cell lysates were immunoblotted with antibodies against RANTES or α-tubulin, respectively. Representative Western blot (a) and quantitative data of RANTES expression in various groups (b) are presented. *P < 0.05 versus pcDNA3 control (n = 3). (c) Id1 promotes the chemoattraction of tubular epithelial HKC-8 cells to THP-1 monocytes in vitro. Id1-overexpressing HKC-8 cells and controls cells were treated with TNF-α for 24 h. Conditioned media were collected for chemotaxis assay to assess their ability to attract THP-1 cells to migrate across the filter of Transwell chambers. Data are expressed as the percentage of migrated cells in total cells added. *P < 0.05 versus pcDNA3 control (n = 3). (d) Immunohistochemical staining shows an induction of RANTES protein in the degenerated tubules (*) in the obstructed kidneys at 7 days after UUO. Scale bar, 50 μm.