Figure 3. LVs inhibit bacterial population growth.
(A) Incubating both EPEC and native intestinal bacterial cultures with 50 μg LVs/ml reduces OD600 achieved at stationary phase (t = 8 - 16 hr) following log growth (t = 4 - 8 hr). Black lines = EPEC, blue and red lines = native E. coli. (B) Stationary phase EPEC cultures incubated with LVs show a reduction in OD600 compared to untreated samples over the course of 16 hrs, both in cultures with nutrient-depleted media (orange lines) or fresh media (dark red lines). Traces represent averaged recordings from multiple experiments (n = 3 - 8). (C) LVs reduce the number of viable bacteria (CFU), as sampled at the end of cultures started with 2.5 × 104 CFU/ml and incubated for 16 hrs or stationary cultures incubated for 16 hrs. *p < 0.05 vs. untreated.