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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: Curr Biol. 2012 Mar 8;22(7):638–644. doi: 10.1016/j.cub.2012.02.030

Figure 1.

Figure 1

Measurement of kinetochore-microtubule dynamics in cells overexpressing Mad2. (A) DIC and time-lapse fluorescent images of prometaphase and metaphase spindles in untreated (control) and Mad2 overexpression (Mad2OX) in PA-GFP tubulin expressing RPE1 cells before (Pre-PA) and at indicated times after photoactivation. Mad2 overexpression is visualized by mCherry fluorescence. (B) Normalized fluorescence intensity over time after photoactivation of spindles in untreated (white circles) and Mad2OX (filled circles) in prometaphase and metaphase cells. Data represents mean ± s.e.m., n≥10 cells.