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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Immunother. 2012 May;35(4):309–320. doi: 10.1097/CJI.0b013e31824b3b14

Figure 5. Effects of NE and P3 on activation of normal mDCs and B-cells.

Figure 5

(A) Normal immature mDCs (CD11c+/CD14−) were cultured up to 6 hours with soluble NE or P3 (5 µg/mL), irradiated leukemia or granulocytes at 1:1 DC to irradiated-cell ratio, or with TNF-α (10 ng/mL) as a positive control. Cells were stained for CD83 or HLA-DR. Results represent 5 experiments performed in duplicate. (B) Normal CD19+ B-cells were enriched from healthy donor blood and cultured for 72 hours with NE or P3 (5 µg/mL), irradiated leukemia or granulocytes at 1:1 B-cell to irradiated-cell ratio, or with CD40L (500 ng/mL)/E (1 µg/mL) as a positive control. Cells were then stained for HLA-DR. Results represent 3 experiments performed in duplicate. All data points represent mean fold change ± SD in MFI of CD83 or HLA-DR compared with unpulsed cells. ANOVA followed by Tukey tests were performed using Prism 5.0 software (*P<0.05).