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. 2012 Mar 6;135(4):1180–1196. doi: 10.1093/brain/aws022

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© The Author (2012). Published by Oxford University Press on behalf of the Guarantors of Brain.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HSJ1a associates with K63 ubiquitylated mutant huntingtin inclusions. Western blots probed with S830, S653 or α-Ub antibodies after HSJ1 (S653 or α-myc) immunoprecipitation of material from 15-week R6/2 brain lysates incubated with (A) purified HSJ1a or (B) lysates from SK-N-SH cells expressing wild-type or UIM inactive (ΔUIM) HSJ1a in the presence of UbK48 chains or UbK63 chains (A and B) (asterisks denote IgG bands). (C) Immunofluorescence images from the cortex of 15-week-old double transgenic mice stained with anti-huntingtin (S830), anti-HSJ1a (16321) and anti-UbK63 antibodies. Scale bars: 10 µm, arrowheads show the position of cytoplasmic inclusions; arrows show the position of nuclear inclusions.