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. 2012 Mar 14;135(4):1115–1127. doi: 10.1093/brain/aws036

Figure 2.

Figure 2

Oxidative stress and increased oxidative markers in ryr zebrafish. Oxyblot analysis of total protein extracts from 7 days post-fertilization control (CTL) and ryr (RYR) zebrafish. (A) Representative blot from single pools of fish (n = 40). Oxyblot ( ± 2,4-dinitrophenylhydrazine reagent) is left, western blot using actin antibody right. (B) Quantitation of oxyblot data expressed as a ratio of oxyblot band average intensity to actin band intensity. Data are from three independent experiments. CTL = 2.5 ± 0.1 versus RYR = 5.7 ± 0.2, P = 0.0001. (C) DCFH assay as performed on pools of 7 days post-fertilization zebrafish (40 fish per pool) from the following strains: control clutchmates (ctl), ryr, candyfloss (caf) and sapje (sap). Data are expressed as fold-change from control values. ryr = 2.1, caf = 1.5 and sap = 1.2. P-values = 0.0005 (*ryr versus ctl), 0.03 (**ryr versus caf), 0.02 (***ryr versus sap), 0.006 (caf versus ctl) and 0.17 (sap versus ctl).