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Functional validation of candidate tumor suppressors. Ectopic expression of candidate tumor suppressors by retroviral (A) or lentiviral (B) vectors in colon cancer cell lines leads to decreased soft agar growth. Triplicates from 2 separate experiments, 2-tailed Student t test, compared with none, mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant.
