Table 2.
Details of Multiplex PCR and SNaPshot Reaction Products
| Mutation | Nucleotide change | PCR fragment (bp) | SNaPshot primer orientation | SNaPshot result (N/M) | SNaPshot fragment N (bp) | SNaPshot fragment M (bp) |
|---|---|---|---|---|---|---|
| FXIII V34L | G/T | 331 | R | C/A | 21 | 22 |
| MTRR A66G | A/G | 129 | F | A/G | 22.6 | 24.5 |
| FGB -455G/A | G/A | 222 | R | C/T | 24.4 | 26.6 |
| MTHFR C677T | C/T | 213 | F | C/T | 27 | 37 |
| MTR A2756G | A/G | 383 | F | A/G | 29 | 26 |
| FV Leiden | G/A | 265 | F | G/A | 33 | 34.5 |
| FII G20210A | G/A | 133 | F | G/A | 36 | 38 |
| PAI-1 4G/5G | T/G | 420 | R | A/C | 41 | 40 |
| FV H1299R | A/G | 177 | R | T/C | 46 | 45 |
| MTHFR A1298C | A/C | 314 | F | A/C | 49 | 48 |