FIG. 2.

395A5, but not 395D2, MAbs bind to the basement membrane zone of human skin. Cryosections of normal human skin were incubated with MAbs 395A5, 395D2, IgELB4 (IgE isotype control), a BP serum (diluted 1:10), a BP180 specific IgG MAb (HD18), or a murine IgG isotype control. Antibody binding to the BMZ was visualized with anti-mouse IgE, anti-mouse IgG, anti-human IgE, or anti-human IgG, as indicated. Antibody binding to BP180 in human skin was evidenced by linear staining at the basement membrane zone (arrows) using 395A5 MAbs, BP sera, and HD18, but was absent with the 395D2 MAb or the isotype controls.