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. 2012 Apr;31(2):111–117. doi: 10.1089/hyb.2011.0102

FIG. 4.

FIG. 4.

395A5 and 395D2 MAbs mediate NC16A-specific histamine release. RBL-SX-38 basophils were coated with 395A5, 395D2, or IgELB4 MAbs or IgE purified from two BP patients (BP IgE1 or BP IgE2) or normal control serum (NH IgE) for 30 min and washed, and degranulation was triggered by exposure to 100 nM NC16A or GST control protein for 30 min. (A) MAbs 395A5 and 395D2 triggered greater histamine release in response to NC16A protein, but not GST control protein, similar to BP IgE1 and IgE2 (B). Control antibodies did not trigger NC16A-specific histamine release.