FIG. 2.

Immunofluorescence staining (IFA) of JEV genotype 3 strain-infected Hela cells with different MAbs. At 48 h post-JEV infection, cells were washed with PBS three times and fixed with 100% paraformaldehyde for 10 min. After being blocked with PBSA for 30 min at 37°C, cells were washed with PBS, reacted with a 1:100 dilution of MAb for 1 h, and incubated with Alexa Fluor 488 goat anti-mouse IgG (H+L) for another 30 min. Cells were then washed three times with PBS and photographed using a fluorescence microscope. IH7 and 2D4 against NS3; 3H7, 3C4, and 3F10 against NS5; PC, JEV polyclone antibody control; cell control, uninfected Hela cells control (200x).