Figure 2.

LPS-mediated lung injury is not attenuated by antagonists to P-selectin or by DNase treatment. Mice were treated with antibodies to P-selectin (30 μg) or GPIIb/IIIa (100 μg), or DNase (1 μg) before LPS inhalation, and killed 4 hours later. (A) Quantification of intravascular (top), interstitial (middle), and alveolar neutrophils (bottom). (B) Protein concentration (top), fluorescein isothiocyanate (FITC)–dextran clearance (middle), and elastase (bottom, uniform bars) and myeloperoxidase (MPO) activity (bottom, hatched bars) in bronchoalveolar lavage fluids. n = 8–10 for each bar. Statistical significance was tested using one-way analysis of variance with Dunnett post hoc test. *Indicates significant difference compared with LPS-treated animals.