Figure 5.

Acid- or sepsis-induced acute lung injury (ALI) is abrogated by disrupting CCL5–CXCL4 heteromers. ALI was induced by intratracheal acid application (Acid) or by cecal ligation and puncture (CLP). MKEY (50 μg) was injected 1 hour before or 1 hour after ALI induction. Mice were killed 4 (Acid) or 24 hours (CLP) after ALI induction. (A) Displayed are intravascular (top), interstitial (middle), and alveolar neutrophil counts (bottom). (B) Protein concentration (top), fluorescein isothiocyanate (FITC)–dextran clearance (middle), and elastase and myeloperoxidase (MPO) activity (bottom) in bronchoalveolar lavage fluids. n = 7 for each bar. Statistical significance was tested using Kruskal-Wallis test with Dunn post hoc test. *Indicates significant difference compared with ALI control mice.