Figure 11.

Activation of p75 increases M-current amplitude without altering voltage dependence of activation. A, Plots of muscarine difference current amplitude versus test step [measured over the end of the step (gray rectangle in Fig. 10D)] show that application of 100 ng/ml of the p75 agonist BDNF (open triangles, n = 6) caused an increase in M-current amplitude compared with saline (filled circles, n = 7; p < 0.05, two-way ANOVA, followed by the Tukey test). B, Plots of normalized tail current amplitude with steps back to −30 mV (measured at dashed line in Fig. 10D) show that the voltage dependence of activation of the M-current for cells recorded from in saline (n = 7) and BDNF (n = 6) did not differ (Boltzmann parameters, V_½ −33.1 ± 1.2 mV vs −29.6 ± 2.3 mV and τ 7.2 ± 0.4 ms vs 8.6 ± 1.2 ms, for saline and BDNF, respectively). Boltzmann fits are shown graphed as solid lines.