Table 1.
Oligonucleotides used in this study
| Fragment/gene | Sequence of sense primer | Sequence of the reverse primer |
|---|---|---|
| Construction of library insert fragments | ||
| 48a | GGATCCattgtttttcctcacagttcgtttt | TCTAGAattgtagcctccgtggcccat |
| 48b | GGATCCtatgttgagtatttttaaaccagcg | TCTAGAtcccgccgccgacgttatgc |
| 48c | GGATCCattgtttttcctcacagttcgtttt | TCTAGAtcccgccgccgacgttatgc |
| 134a | CGGGATCCcgttattcttaaaaatgagcgtaa | CGTCTAGAtcataacggcgaagttagcg |
| 134b | CGGGATCCccggcgaagcgg | CGTCTAGAaataacgggtaatgtcatttgttt |
| 134c | GATCtaacttcgccgttatgatcggtcgtcttttaagcaac tattgacacacac | CTAGgtgtgtgtcaatagttgcttaaaagacgaccgatca taacggcgaagtta |
| 134d | GATCggttaaggtcaaagaaagacacgttctgataattctt acttgtcattcgctaacttcgccgttatgatcggtcgtc ttttaagcaactattgacacacac | CTAGgtgtgtgtcaatagttgcttaaaagacgaccgatcat aacggcgaagttagcgaatgacaagtaagaattatcaga acgtgtctttctttgaccttaacc |
| 134e | CGGGATCCcgttattcttaaaaatgagcgtaa | CGTCTAGAgtgtgtgtcaatagttgcttaaaagacgaccg atcataacggcgaagttagcg |
| 212a | GATCctttcattcgaaagtaatttaatctttatatga aataagagaggccgttt | CTAGaaacggcctctcttatttcatataaagattaaattac tttcgaatgaaag |
| 212b | GATCtaacgcctctttgtcagaacctctccattcgttgac gcacatcaagatagctttcattcgaaagtaatt taatctttatatgaaataagagaggccgttt | CTAGaaacggcctctcttatttcatataaagattaaattac tttcgaatgaaagctatcttgatgtgcgtcaacgaat ggagaggttctgacaaagaggcgtta |
| 301a | GGATCCggcccatattttatttagaggtaaac | TCTAGAattgtttttcctcacagttcgttttt |
| 301b | GGATCCtgaaaactcgcgactcgcaaac | TCTAGAatccgttttaaaatctggttttcac |
| 301c | GGATCCggcccatattttatttagaggtaaac | TCTAGAatccgttttaaaatctggttttcac |
| qRT-PCR | ||
| mltD | TGCCAGACGTCTCAGAACAC | CATCGTGCCTGACTCGTAAA |
| PflE | ATTTTCTGCCCTACCACACG | GCGTACTTCTGGGCAAAATC |
| glpA | CTGGTACCTGGCGATACGAT | ACGCAGCAGGATATCGACTT |
| glpT | CAACGAAAAAGCGGAAGAAG | GCGCAGCAGATAAACAAACA |
Listed are oligonucleotides for the construction of library insert fragments and for qRT-PCR.