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. 2011 Dec 5;40(7):2884–2897. doi: 10.1093/nar/gkr1066

Figure 1.

Figure 1.

Distribution of MeCP2in HeLa cells in response to treatment with DNA methylating and de-methylating agents. (A) Western (MeCP2) blot analysis of the S1, SE and P chromatin fractions obtained from the nuclei of HeLa S3 cultures grown in the absence N (native) or in the presence of: 2 mM 3-ABA, methylated (ABA); 1 µM 5-aza-2′-deoxycytidine, de-methylated (Aza). Coomassie blue-stained or antibody detected histone H4 (H4) was used for normalization purposes. (B) Western blot analysis of the S1, SE and P chromatin fractions obtained from the nuclei of mouse 3T3 fibroblast cultures grown in the absence N (native) or in the presence of: 2 mM 3-ABA, methylated (ABA); 1 µM 5-aza-2′-deoxycytidine, de-methylated (Aza). The upper part corresponds to the MeCP2 western and the lower part is a Comassie blue stained of the SDS–PAGE after electroblotting. CM: Chicken erythrocyte histone marker. (C) HPLC-determined relative meC/C percentile of the mouse 3T3 fibroblasts used for the analysis shown in (B).