Figure 6.

MeCP2 is associated with mononucleosomes containing histone H2A.X and histone H3 PTMs. (A–D) Mononucleosomes from S1 and SE fractions were coimmunoprecipitated under native conditions using a MeCP2 antibody. The immunoprecipitated nucleosomes were then analyzed by western blot using the antibodies indicated. Normal rabbit IgG was used as a non-specific control. The pull-downs for both the MeCP2 antibody and IgG were compared to a normalized initial sample input used in the coimmunoprecipitations. (E) MeCP2 pull-down experiments using H3 N-terminal peptides with several PTMs. (1) MeCP2 western blot analysis after pull-down using biotinylated peptides and streptavidin beads. (2) Quantification of the results in (1). Results are expressed as percent of MeCP2 eluted compared to total input. Error bars represent standard error of the mean of triplicate experiments.