Figure 4.

Disruption of the Cdt1–Mcm6 interaction by mutations on their interacting surfaces impaired pre-RC formation and DNA replication. (a) Wild-type and mutants of hMBD were pulled down by GST-tagged wild-type hCBD and visualized by Coomassie Blue staining. (b)Chromatin loading of Mcm2 (S. cerevisiae) is interrupted by the Mcm6-5A mutant (Supplementary Material). (c) Chromatin loading of Mcm2 (S. cerevisiae) is interrupted by the Cdt1-3A mutant (Supplementary Data). (d) Mcm6-5A cells arrest in S-phase with large buds (S. cerevisiae) (Supplementary Data). Flow cytometry was performed for the cell samples taken at the indicated time points. % Bud. percentage of budding cells. (e) Cdt1-3A cells arrest in S-phase as in (d).