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. 2001 May 8;98(10):5625–5630. doi: 10.1073/pnas.091610798

Figure 1.

Figure 1

Sequence alignment and two-hybrid assays. (A) Sequence alignment between yeast Rck2 and mammalian MAPKAP kinases. Phosphorylation sites for proline-directed kinases are indicated by arrows. MAP kinase-binding domain at the C terminus. (B) Mapping of the MAP kinase-binding domain in Rck2. ATP, ATP-binding domain; P, potential phosphorylation sites; BD, MAP kinase-binding domain. Different truncated forms of Rck2 shown (Left) were tested, and the resulting two-hybrid interaction is displayed (Right).