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. 2001 May 8;98(10):5625–5630. doi: 10.1073/pnas.091610798

Table 1.

Results of the two-hybrid screen with Hog1 as a bait

Gene Number of clones
2-H interaction
Total Different HIS3 Lac-Z
RCK2 12 4 +++ +++
RNR4 2 2 ++ ++
GLO3 1 1 ++ ++
ADH3 2 2 ++ ++
others 8 7 ++ ++

A yeast cDNA library was screened with Hog1p as bait. Transformants (560,000) were screened, and clones, which were positive for lac-Z and HIS3 reporter genes, were sequenced.