Table 1.
Gene | Number of clones
|
2-H
interaction
|
||
---|---|---|---|---|
Total | Different | HIS3 | Lac-Z | |
RCK2 | 12 | 4 | +++ | +++ |
RNR4 | 2 | 2 | ++ | ++ |
GLO3 | 1 | 1 | ++ | ++ |
ADH3 | 2 | 2 | ++ | ++ |
others | 8 | 7 | ++ | ++ |
A yeast cDNA library was screened with Hog1p as bait. Transformants (560,000) were screened, and clones, which were positive for lac-Z and HIS3 reporter genes, were sequenced.