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. 2011 Dec 2;40(7):3218–3231. doi: 10.1093/nar/gkr1139

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Binding of 6-nt RNA oligomers to individual RBDs of Msi1. Overlays of the ¹⁵N-HSQC spectra sections of RBD1 (red), and RBD1 in the presence of 0.2 (orange), 0.5 (green), 1.0 (blue) and 1.2 (purple) equivalents of 6-nt RNA oligomers: (A) r(UAGGUA) (numb6-1); (B) r(GGUAGU) (numb6-2); (C) r(UAGUAG) (numb6-3); (D) r(GUAGUU) (numb6-4); (E) r(AGUUUU) (numb6-5) and (F) r(GUUUUA) (numb6-6). Overlays of the ¹⁵N-HSQC spectra sections of RBD2 (red) and RBD2 in the presence of 0.2 (orange), 0.5 (green), 1.0 (blue) and 1.2 (purple) equivalents of 6-nt RNA oligomers: (G) numb6-1; (H) numb6-2; (I) numb6-3; (J) numb6-4; (K) numb6-5 and (L) numb6-6. (M) The chemical shift changes, Δδ, were obtained by subtracting the chemical shifts of Msi1 backbone ¹H^N for the free protein from the chemical shifts of Msi1 backbone ¹H^N in the complex (Msi1:RNA oligomer = 1:1).