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. 2011 Dec 2;40(7):3218–3231. doi: 10.1093/nar/gkr1139

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — r(GUAGU) (numb5) and r(GUAGUU) (numb6-4) bind equally well to RBD1 of Msi1. (A) Overlay of the ¹⁵N-HSQC spectra of RBD1 (red), RBD1 in the presence of a 1.0 equivalent of numb5 (cyan) and RBD1 in the presence of a 1.0 equivalent of numb6-4 (black). (B) The chemical shift changes, Δδ_H (Δδ_N), were obtained by subtracting the chemical shifts of Msi1 backbone ¹H^N (¹⁵N) for the free protein from the chemical shifts of Msi1 backbone ¹H^N (¹⁵N) in the complex.