Table 2.
Structural statistics for the Msi1 RBD1:numb5 complex
| Restraints for final structure calculations | |
|---|---|
| Upper distance limits | |
| Total | 1790 |
| Intra-protein | 1716 |
| short-range, |i−j|≤1 | 835 |
| medium-range, 1<|i−j|<5 | 281 |
| long-range, | i−j |≥5 | 600 |
| Intra-RNA | 16 |
| Inter-molecular | 58 |
| Number of dihedral angle restraints | |
| χ angles | 4 |
| Number of hydrogen bond restraints | 0 |
| Structure statistics (20 structures) | |
| Number of NOE violations >0.2Å | 0 |
| Number of dihedral angle violations >5° | 0 |
| Average CYANA target function (Å): | 0.18 |
| Average AMBER energy (kcal·mol−1): | −4059.47 |
| Average AMBER total restraint violation (kcal·mol−1) | 4.27 |
| RMS deviation from the mean coordinate structure | |
| Backbone heavy atoms (Å) | |
| Protein (21–97) | 0.33 ± 0.09 |
| All heavy atoms (Å) | |
| Protein (21–97) | 0.96 ± 0.11 |
| Protein (21–97) and RNA | 0.94 ± 0.11 |
| Ramachandran statistics (%) | |
| Residues in most favored regions | 87.6 |
| Residues in additional allowed regions | 11.6 |
| Residues in generously allowed regions | 0.3 |
| Residues in disallowed regions | 0.5 |