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. 2011 Dec 1;40(7):2846–2861. doi: 10.1093/nar/gkr1141

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — SQ18, SQ893 and SQ485 sRNAs controlled the gbs0031, gbs1263 and gbs1588 target genes expression, respectively. (A) Quantitative real-time RT–PCR analysis of expression of gbs0031, gbs1263 and gbs1588 gene. The relative expression of the three mRNA genes were determined by comparing over-expressing strains S. agalactiae NEM316 + pTCV-SQ18 or pTCV-SQ485 or pTCV-SQ893 against the wild-type S. agalactiae NEM316 isolate. (B) Analysis by Western blot and quantitative RT–PCR of the expression of the gfp and SQ18 gene expression in E. coli TOP10 strain harboring pZE2R-SQ18 or mock plasmids combined with pXG-0 (no gfp target control) or pXGgbs0031::gfp expression plasmids. SQ18 expression was normalized to 1.0 for the TOP10 + pZE2R-SQ18 + pXG-0 strain. Asterisks indicate a significant difference between mean values in unpaired t tests (P < 0.01).