Table 5.
Yeast agglutination assays for E. coli 536 derivatives
| Strain | Yeast agglutination titer |
|---|---|
| 536 + pZE2R-null | 1/16 |
| 536 + pZE2R-fimR | 1/64 |
| 536 Δfim::cat + pZE2R-null | NO |
| 536 Δfim::cat + pZE2R-fimR | NO |
| 536 + pZE21-null | 1/16 |
| 536 + pZE21-antifimR | 1/4 |
| 536 Δfim::cat + pZE21-null | NO |
| 536 Δfim::cat + pZE21-antifimR | NO |
| 536 | 1/16 |
| 536Δhfq::KmFRT | NO |
The level of expression of type 1 fimbriae was assessed in E. coli 536 wild type and mutant strains expressing the FimR sRNA, the antiFimR sRNA or mock plasmids. No 536 Δfim strains agglutinated yeasts indicating that the agglutination phenotypes resulted from the expression of type 1 fimbriae. NO: not observable.