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Detection of subtelomeric RNA. (A) Schematic representation of the ends of Chromosomes I and II. Telomeres (Telo) are ∼270 bp in length and the subtelomeric elements are ∼1.8 kb (for STE1) and ∼5 kb (STE2) in length. Probes are indicated by black solid lines. (B) Northern blot analysis was performed as in Figure 1B, except that a subtelomeric sequence (STE1) probe was used. RNase-sensitive bands of ∼0.4, 0.6, 0.8 and 1.2 kb are detected in wt and taz1Δ but not Otrt1Δ samples.
