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SRSF2:RNA UV cross-linking. Various purified proteins (BSA, GB1-6His-SRSF2 9-101 wild-type and point mutants) were incubated with 32P-labelled 9-mer RNA (AGCAGAGUA) before binding reactions were irradiated with UV (+) or not (−) and analysed by 15% SDS–PAGE stained with Coomassie and autoradiography.
