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. 2011 Dec 7;40(7):3006–3017. doi: 10.1093/nar/gkr1197

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Ribosome occupation of mRNAs for specific proteins is accelerated upon ascorbate treatment in a p180-dependent manner. HEL cells were cultured in the presence or absence of ascorbate and treated with a control siRNA or p180-specific siRNA. The membrane fractions obtained by sequential digitonin extractions were subjected to polysome analyses using a 15–50% sucrose gradient. cDNAs were synthesized from RNA samples extracted from individual tubes. The distributions of mRNAs from non-stimulated cells with cont-si, ascorbate-treated cells with cont-si and ascorbate-treated cells with p180-si are plotted. Relative signal intensity of mRNAs for procollagen type 1 alpha chain (A), fibronectin (B), MMP-2 (C) and TIMP-1 (D) are shown. M, monosome fraction; P, polysome fraction.