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. 2011 Dec 10;40(7):3289–3298. doi: 10.1093/nar/gkr1209

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Labeling and removal of Cy5 fluorophores from protein markers via strand displacement reactions of a three-strand probe complex (PC_3s). (A) Selective labeling of expressed GFP proteins in CHO cells. The OFF reactions are now efficient, and yield a signal (cell intensity) to background (slide surface intensity) ratio of 1.08. The ratio of labeled/erased probe intensities, or ON/OFF ratio, is 28.6. This result is reflected in (B) pixel intensities for cross sections as well as (C) histogram of average, whole-cell Cy5 signal intensities for 20 cells in their ON and OFF states.