Figure 5.

P68 and p72 facilitate RNA foci formation. Human muscle cells expressing 960-interrupted CTG repeats under the control of a Tet-on inducible promoter was used to attend the early steps of RNA foci formation. The number of nuclei containing foci was determined at different times after doxycycline addition (1, 3, 6 and 24 h) in non-treated or treated cells by siRNA to inhibit p68/p72. SiRNA duplexes were transfected 48 h before CUG repeats induction by doxycycline. Quantification results from three independent experiments. Approximately 300 cells were counted for each condition, with error bars indicating standard deviation *P < 0.05, **P < 0.01, ***P < 0.001.