Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 9;40(7):3159–3171. doi: 10.1093/nar/gkr1228

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — p68 regulates splicing of TNNT2 alternative exon 5. (A) Western blot analysis of human myoblasts treated with a control luciferase siRNA, (siLuc), sip68/p72 or siMBNL1. About 15 µg (lanes 1, 3 and 5) or 7.5 µg (lanes 2, 4 and 6) of proteins were loaded per lane. The blot was probed with antibodies against p68, MBNL1 and emerin as a control. (B) RT–PCR analysis of endogenous transcripts in human myoblast cells transfected with a control siRNA (siLuc), siRNA against p68/p72 or siRNA against MBNL1. The histogram shows the quantification of exon inclusion. Results are derived from six independent experiments for TNNT2 and four independent experiments for INSR, MBNL1 and ATP2A1 with error bars indicating standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.