Table 1.

Observed affinity of CRP to various ICAP probes from this and previous studies with indicated amounts of cAMP

Source	Method	Probe	[cAMP] (M)	Kd (M) (± SEM)
(8)	Filter binding	ICAP oligo 32P	2 × 10−4	1.4 ± 0.3 × 10−11a
(8)	Filter binding	ICAP oligo 32P	0	>1.0 × 10−7a
This study	DRaCALA	ICAP oligo 32P	2 × 10−4	3.6 ± 0.4 × 10−11
This study	DRaCALA	ICAP oligo 32P	0	> 1.0 × 10−6
This study	DRaCALA	ICAP* plasmid 32P	2 × 10−4	4.1 ± 1.0 × 10−11
This study	DRaCALA	ICAP* plasmid 32P	0	>1.0 × 10−6
This study	EMSA	ICAP oligo 32P	2 × 10−4	8.1 ± 0.8 × 10−11

All reported K_d values from this study were determined by DRaCALA and the standard error of three trials is reported.

^aThe affinity reported in the Gunasekera et al. paper is the binding constant or association constant K_a. We have taken the inverse of those values to give the K_d reported in Table 1 to match our K_d measurements.